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KMID : 0545119980080010061
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 1 p.61 ~ p.66
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Biodegradation of Phenol by a Trichloroethylene-cometabolizing Bacterium
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Park, Geun Tae
Son, Hong Joo/Kim, Jong Goo/Lee, Sang Joon
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Abstract
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A microorganism which degrades phenol and co-metabolizes trichloroethylene (TCE) was isolated from Yangsan stream after enrichment in a medium containing phenol as the sole carbon source. The isolate EL-43P was identified as the genus Rhodococcus by its morphological, cultural and physiological characteristics. Phenol-induced cells of Rhodococcus sp. EL-43P degraded TCE. Toluene and nutrient broth could not replace the phenol requirement. The optimal conditions of initial pH and temperature of media for growth were 7.0∼9.0 and 30∼50℃, respectively. Rhodococcus sp. EL-43P could grow with phenol up to 1,000 ppm. Growth was inhibited by phenol at a concentration above 1,50O ppm. It was observed that Rhodococcus sp. EL-43P was able to degrade 90% of phenol (1,000 ppm) after 40 h in a culture. Phenol-induced cells of Rhodococcus sp. EL-43P degraded 95% of 5μM TCE in 6 h. Rhodococcus sp. EL-43P hardly degraded TCE above 100μM.
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